Bio-Rad PharosFX™ and PharosFX Plus Systems - Manual (Page 3)

Table of Contents
Section 1 General Information ......................................................................1
1.1 About this Manual ......................................................................................1
1.2 Safety Information......................................................................................1
1.2.1
General Cautions ...........................................................................1
1.2.2 General Warnings ..........................................................................2
1.2.3 Power Safety Information ...............................................................2
1.2.4 Laser Safety Information ................................................................3
1.2.5 Screen Eraser Safety Information...................................................4
Section 2 Introduction ...................................................................................4
2.1 System Capabilities ...................................................................................4
2.2 System Description....................................................................................4
2.3 Theory of Operation ...................................................................................8
2.3.1
Fluorescence Detection Mechanism...............................................8
2.3.2 Storage Phosphor Detection Mechanism .......................................9
2.3.3 Data Processing and Analysis........................................................9
2.4 Overview of the Imaging Process.............................................................10
2.4.1
Steps in Fluorescence Imaging ....................................................10
2.4.2 Steps in Storage Phosphor Imaging .............................................10
Section 3 System Installation......................................................................11
3.1 Operating Requirements..........................................................................11
3.1.1
System Location...........................................................................11
3.1.2 AC Power Requirements..............................................................12
3.1.3 Host Computer Recommendations...............................................13
3.2 System Setup ..........................................................................................13
3.2.1
Shipping Check ............................................................................13
3.2.2 Unpacking ....................................................................................13
3.2.3 Electrical and Communication Connections..................................15
3.2.4 Software Installation .....................................................................16
Section 4 System Operation ......................................................................16
4.1 Starting the Scanner ................................................................................16
4.2 Fluorescence Operating Procedures........................................................17
4.2.1
Sample Preparation .....................................................................17
4.2.2 Inserting a Fluorescent Sample into the Scanner .........................18
4.2.3 Scanning Fluorescent Samples ....................................................19
4.2.4 Inserting Emission Filters .............................................................20
4.3 Fluorescence Imaging Using the External Laser Module..........................22
4.3.1
External Laser Safety Information ................................................23
4.3.2 Fiber Optic Cable Alignment.........................................................23
4.4 Phosphor Imaging Operating Procedures ................................................24
4.4.1
How to Prepare K-Type Imaging Screens ....................................24
4.4.2 How to Erase Imaging Screens ....................................................24
4.4.3 How to Prepare Samples .............................................................25
4.4.4 How to Use the Exposure Cassette ..............................................27
4.4.5 How to Scan the Imaging Screen .................................................28