Table 4.2 – Bio-Rad PharosFX™ and PharosFX Plus Systems User Manual

Page 26

The standard emission filter configurations are as follows:

Table 4.2

Note: When a 488 Excitation Option is ordered an additional 530 Bandpass Filter is
supplied (e.g. FITC and Cy2). When a 635 nm Excitation Option is ordered an additional
695 Bandpass Filter (e.g. Cy5) is supplied. The recommended filter positions are indicated
in table 4.2 above.

4.3 Fluorescence Imaging Using the External Laser Module

The optional External Laser Module can be attached to PharosFX and PharosFX Plus to
expand the system's range of laser excitation sources. The installation of the External
Laser Module requires a field service engineer, please arrange the visit through Technical
Support (1-800-424-6723 press 2 for Technical Support). The standard external module
contains an Argon Ion laser, which is ideal for imaging fluorophores excited by 488 nm
light. The External Laser Module can also be outfitted with an additional 635 nm laser,
which further adds to the flexibility of the PharosFX imaging system.

The PharosFX Scanner detects when the External Laser Module is attached and the
External Laser will be used whenever the appropriate application is selected.

532

only

532 &
488

532, 488 & 635

PharosFX

Filter

Wheel A B

B A B

Blank

605 nm

Blank

605 nm

Blank

605 nm

2 Blank Blank 2 Blank

Blank 2 Blank

695 nm

640 nm

Blank 3

640 nm

530 nm

640 nm

530 nm

4 Blank Blank

4 Blank

Blank

4 Blank Blank

532 only

532 &
488

532, 488 & 635

PharosFX Plus

Filter

Wheel A B

B A B

Blank

605 nm

Blank

605 nm

Blank

605 nm

390 nm

Blank 2

390 nm

Blank 2

390 nm

695 nm

640 nm

BP Blank

640 nm

530 nm

640 nm

530 nm

4 Blank Blank

4 Blank

Blank

4 Blank Blank

filter can not be changed.