Bio-Rad PharosFX™ and PharosFX Plus Systems - Manual (Page 31)

Plastic wraps will attenuate weak beta radiation signals according to the following table.
Table 4.4 Attenuation of radiation by various plastic wraps.
Note: When enclosing a sample in plastic wrap or a heat-sealable bag, make sure that
there are no large air bubbles or surface wrinkles on the side of the sample that will come
into contact with the screen. Air bubbles and wrinkles will prevent close contact with the
screen, which may result in poor image quality and reduced sensitivity.
Note: Ensure that the external surface of the wrap or heat-seal bag is wiped dry to minimize
potential screen contamination.
4.4.4 How to Use the Exposure Cassette
Exposure Cassette-K
Fig. 4.12. Steps in use of the Exposure Cassette-K.
1. Push the two release buttons away from the edge of the cassette to open.
2. Use a clean cloth to wipe the internal surfaces of the cassette and remove any
contamination.
3. Tape the prepared sample with the active surface facing upwards to the grid marked
exposure pad, making sure to align the sample straight and in the correct orientation. It
is recommended that plastic wrap is then taped in place over the sample.
Wrap Type 32 P 14 C 3 H Application
2 mm Seal-A-Meal Bag 16% 82% >99.9% Wet samples
0.5 mm Saran Wrap 6% 50% 99.7% Moist samples
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