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Additional materials and equipment required – Bio-Rad Experion Protein Analysis Kits User Manual

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Additional Materials and Equipment Required

Experion automated electrophoresis station

Experion priming station

Microcentrifuge (1,000–10,000 x g)

Aluminum foil

Heating block or water bath set at 95–100°C

Benchtop vortexer

Extra spin filter for additional filtration steps (optional, catalog #700-7254)

Ice

The Experion Pro260 Starter Kit Tests

The Experion Pro260 starter kit includes all the reagents, consumables, and instructions
required to perform three tests, each of which demonstrates a key protein analysis function
of the Experion system. Each test uses various dilutions and preparations of a standard
protein, bovine g-globulin (BGG), and each test follows the standard Experion Pro260
assay workflow, which is summarized below.

Experion Pro260 Starter Kit Tests

Test Name

Purpose

Description

1

Sizing and

Used for familiarization with the

BGG samples are analyzed using

relative

Experion system and for

standard nonreducing conditions

quantitation

troubleshooting. Demonstrates

and are quantitated using relative

the basic functions of a Pro260

quantitation against the upper

assay and how to perform relative

marker

quantitation, the easiest, fastest
method of protein concentration
determination. Also demonstrates
protein sizing and how to
determine the accuracy and
reproducibility of a Pro260 analysis

2

Percentage

Used for demonstration of the

BGG samples are analyzed under

determination

percentage determination function

reducing conditions to allow

of Experion software, which can be

separation of the BGG subunits. The

used for rapid evaluation of sample

heavy- and light-chain fragments are

purity

used to demonstrate the percentage
determination calculations

3

Absolute

Used for demonstration of the

Five BGG samples at various

quantitation

absolute quantitation method, which concentrations are used to generate

using a

involves creation of a calibration

the calibration curve used for

calibration

curve. Demonstrates how to

quantitation of another BGG sample

curve

generate and evaluate the linearity
of a calibration curve and how to
determine the accuracy and
reproducibility of absolute
quantitation. The results
demonstrate how use of a
calibration curve can
significantly improve
quantitation accuracy

3

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