Bio-Rad Experion Protein Analysis Kits User Manual
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8.
Click the Protein Search Results tab and scroll down to find the %CV calculation. In
the example given in Figure 1.10, the %CV is 1.55%.
Fig. 1.10. Results table from Test 1 showing reproducibility of protein sizing. In this example, the
%CV for sizing is 1.55%. Note that the apparently large %CV for concentration is misleading (different
sample concentrations were analyzed).
9.
To perform a qualitative evaluation of quantitation reproducibility, overlay the
electropherograms of replicate samples. For example, in the virtual gel, select lane 3
and hold the Ctrl key while selecting lanes 6 and 9. The electropherograms from these
wells (1,000 ng/µl samples) overlap (Figure 1.11). Tighter overlaps signify better
reproducibility. Repeat this step for the other two concentrations examined.
Fig. 1.11. Electropherogram overlay of the 1,000 ng/µl samples. The virtual gel (right) shows that the
samples in wells 3, 6, 9 are being compared.
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