Bio-Rad Experion Protein Analysis Kits - Manual (Page 5)

Introduction — Product Description and Components
Experion ™ System and Pro260 Starter Kit
The Experion automated electrophoresis system employs LabChip microfluidic technology
to automate protein and nucleic acid electrophoresis and analysis. This system integrates
separation, detection, and data analysis within a single platform and uses smaller sample
and reagent quantities than standard analysis methods. The Experion system accomplishes
analysis in a single 30 minute, automated step.
The Experion electrophoresis system includes the following components:
• Automated electrophoresis station
• Microfluidic chips
• Priming station
• Vortex station (for RNA and DNA analyses only)
• Reagents (analysis kits and individual components)
• Experion software
The Experion system includes the following components: 1) automated electrophoresis station, 2)
priming station, 3) vortex station used for nucleic acid analysis only, 4) system operation and data analysis
tools, and 5) analysis kits, which include the (a) chips and (b) reagents for protein (Pro260 kit),
standard-sensitivity RNA (StdSens kit), high-sensitivity RNA (HighSens kit), and DNA (DNA 1K and 12K
kits) analyses.
At the center of the Experion system is the microfluidic chip, which contains a series of
plastic wells bonded over a small glass plate. The glass plate is etched with an optimized
network of microchannels, which connect with the base of the plastic wells. The channels
are primed with a gel matrix, the samples are applied to the appropriate wells, and the
electrophoresis station directs the samples through these microchannels by controlling the
voltages and currents that are applied. The microfluidic chip, in conjunction with the
Experion reagents, electrophoresis station, and software accomplish separation, staining,
destaining, detection, and basic data analysis without any user intervention.
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