Bio-Rad Experion Protein Analysis Kits - Manual (Page 28)

3.3 Data Analysis
Evaluate the run and the analysis of the data by Experion software.
1. Evaluate the virtual gel to ensure that all lanes (samples) are visible. Check that the
upper and lower markers are present in each sample (indicated by pink triangles) and
that they are aligned across all lanes. Select Graph > Scale to Global to adjust the
virtual gel so that it resembles an SDS-PAGE separation (Figure 3.2).
Fig. 3.2. Virtual gel generated by Test 3. L, Pro260 ladder; lanes 1–3, 9, and 10, BGG sample
(“unknown”); lanes 4–8, BGG calibrants. In this example, the virtual gel was “scaled to global”.
2. Evaluate the separation of the Pro260 ladder. This is a critical step in data analysis
because much of the automated data analysis performed by Experion software is
based on the successful separation of the Pro260 ladder. To display the ladder
electropherogram, click the ladder well in the project tree, or click on the lane labeled L
in the virtual gel. The electropherogram should resemble the one shown in Figure 1.8.
3. Examine the separation of the 1,000 ng/µl BGG sample (S1). Click on the sample
name in the project tree or on the lane in the virtual gel. The electropherogram appears
and should resemble the electropherogram shown in Figure 1.9.
4. Under the Run Info tab, designate the wells that contain the proteins for the calibration
curve (wells 4–8 in the Use as Standard column) and enter their concentrations
(Figure 3.3). Click Apply.
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