Bio-Rad Experion Protein Analysis Kits - Manual (Page 24)

2.3 Data Analysis
Evaluate the run and the analysis of the data by the Experion software.
1. Evaluate the virtual gel to ensure that all lanes (samples) are visible. Check that the
upper and lower markers are present in each sample (indicated by pink triangles) and
that they are aligned across all lanes. Select Graph > Scale to Global to adjust the
virtual gel so that it resembles an SDS-PAGE separation (Figure 2.2).
Fig. 2.2. Virtual gel generated by Test 2. L, Pro260 ladder; lanes 1,3,5,7, and 9, 500 ng/µl BGG; lanes
2,4,6,8, and 10, 1,000 ng/µl BGG. In this example, the virtual gel was “scaled to global”.
2. Evaluate the separation of the Pro260 ladder. This is a critical step in data analysis
because much of the automated data analysis performed by Experion software is
based on the successful separation of the Pro260 ladder. To display the ladder
electropherogram, click the ladder well in the project tree, or click on the lane labeled L
in the virtual gel. The electropherogram should resemble the one shown in Figure 1.8.
3. Examine the separation of 1,000 ng/µl BGG sample (S1). Click on the sample name in
the project tree or on the lane in the virtual gel. The electropherogram appears and
should appear as shown in Figure 2.3. Under reducing conditions, BGG separates into
light- and heavy-chain fragments; therefore, the electropherogram in Figure 2.3 has
more peaks than does the electropherogram in Figure 1.9 (non-reducing conditions).
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