Bio-Rad Experion Protein Analysis Kits - Manual (Page 33)

Virtual gel generated by Experion Pro260 analysis. Shown are separations of the Pro260 ladder (L)
and 10 samples on the same chip.
Once separation is complete, the software subtracts background noise, removes spikes,
identifies and integrates peaks, and assigns their sizes and concentrations. Proteins are
identified as peaks in the electropherograms and as bands in the virtual gel. The results of
data analysis are also tabulated and presented in a Results table at the end of analysis. The
types of data available include the following:
• Protein size (or mass, in kD)
• Protein concentration (in ng/µl) derived by relative and/or absolute quantitation
• % Of total sample (derived by percentage determination)
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