Bio-Rad Experion Protein Analysis Kits - Manual (Page 16)

Fig. 1.6. New Run dialog. The Experion system stops analysis when it reaches the number of samples
entered.
7. Select the number of samples to be analyzed (10 for all three tests).
8. Click Start to begin the chip run. The green LED in the center of the front panel on the
electrophoresis station blinks once the run has started.
Warning: Do not open the lid of the Experion electrophoresis station until the run is
complete. The lid does not lock. Opening the lid prematurely aborts the run.
The system performs a number of checks at the beginning of a run: it confirms that a chip
has been inserted, that all wells contain liquid, that electrical connections are made, etc. A
calibration counter marks the progress of these calibrations in the upper right corner of the
screen.
Note: An “IV Check Error” message indicates the system cannot make electrical contact in
one or more of the wells. This often means that either there is a bubble at the bottom of a
well or that a well is empty. If this message appears, abort the run, and check the chip for
bubbles or empty wells. Refill the affected well(s), and start the run again.
9. Enter the sample names in the Sample Information window. For example, for wells 1,
4, 7, and 10, enter “S3”. In addition, add sample details, such as sample concentration.
Alternatively, enter this information after the run is complete using the Run Info tab.
10. During separation, the sample name is highlighted in gray in the project tree, and the
electropherogram trace and virtual gel bands appear in real time. By default, the
electropherogram of the sample being separated appears in the electropherogram
view; in the gel view, the lane corresponding to that sample is outlined in pink and has
a dark background. To display the electropherogram from another sample that has
already been separated, click on either the sample name in the project tree or on a
lane in the virtual gel.
11. When the analysis is complete (after approximately 30 min), the instrument beeps and
a window opens indicating the end of the run. Select OK to the prompt and remove the
Pro260 chip from the chip platform.
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