Bio-Rad Experion Protein Analysis Kits - Manual (Page 18)

• Flat baseline
• Marker peaks at least 20 fluorescence units above the baseline
Fig. 1.8. Electropherogram showing the separation of the Pro260 ladder. Note the flat baseline and
well-resolved peaks. All identified peaks are numbered, and the lower and upper markers are indicated
by green asterices. The 260 kD protein in the Pro260 ladder is labeled as the upper marker by Experion
software.
4. Examine the separation of the 1,000 ng/µl BGG sample (S1). Click on the sample
name in the project tree or on lanes 3, 6, or 9 in the virtual gel. The electropherogram
appears and should have the following features (Figure 1.9):
• Two well-resolved marker peaks and one set of system peaks
• Sample peaks located between the system peaks and upper marker
• Flat baseline
• Marker peaks at least 20 fluorescence units above the baseline
14
10010510A.qxp 2/13/2008 11:43 AM Page 14