2 kit components, 3 storage conditions, 4 specifications – Bio-Rad Experion Protein Analysis Kits User Manual
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1.2 Kit Components
Table 1.1. Components of the Experion Pro260 analysis kit.
Item
Description
Volume (per Vial)
10-Chip Kit
Pro260 chip
Microfluidic chips used for protein separation
10 chips
Cleaning chip
Chip used for cleaning electrodes
1 chip
Pro260 gel
Proprietary polymeric sieving matrix
520 µl
3 vials
Pro260 stain
Proprietary fluorescent dye
45 µl
1 vial
Sample buffer
Buffer for protein sample preparation; contains a 1.2 kD
400 µl
1 vial
lower marker and 260 kD upper marker for alignment of
samples to the Pro260 ladder
Pro260 ladder
Protein standard containing 9 purified recombinant proteins
60 µl
1 vial
of 10–260 kD and optimized for automated electrophoresis
on the Experion system
Spin filters
Used for filtering reagents during sample preparation
3 filters
1.3 Storage Conditions
Table 1.2. Storage conditions.
Item
Storage
Shelf Life
Experion Pro260 reagents
4ºC
See expiration date on packaging
Experion Pro260 chips
Ambient
See expiration date on packaging
Gel-stain solution (GS, prepared)
4ºC
1 month from filtration (can be refiltered once)
Gel (G, prepared)
4ºC
1 month from filtration (can be refiltered once)
1.4 Specifications
Number of sample wells per chip
10
Sample volume required
4 µl
Total run time
~30 min per chip
Protein sizing range
10–260 kD
Limit of detection
2.5 ng/µl carbonic anhydrase (10 ng total) in 1x PBS; similar to
colloidal Coomassie Blue G-250 stain
Linear dynamic range
5–2,000 ng/µl (bovine serum albumin in 1x PBS)
Experion Pro260 Analysis Kit