Bio-Rad Experion Protein Analysis Kits - Manual (Page 11)

Dislodge bubbles at the bottom of a well with a clean pipet tip, or remove the solution and load it again.
Use a primed and loaded chip within 5 min of loading. When chips are not used within this time,
reagents may evaporate, leading to poor results or a chip performance error.
Fill all the chip wells when running an analysis. Use blank samples (prepared with water instead of
sample) or replicates if necessary. All 16 electrode pins must be in contact with liquid; otherwise, an IV
(current voltage) check failure error will occur.
2.3 Running the Analysis
Place the electrophoresis station on a stable surface, where it will not be subjected to vibrations or other
movement, and away from direct sunlight and all other potential sources of extreme heat.
Power on the electrophoresis station before launching Experion software.
The first time that the Experion electrophoresis station is used, confirm that communication has been
established between the software and electrophoresis station before preparing the reagents.
Do not open the lid of the electrophoresis station during a run. The run will abort if the lid is opened.
2.4 General Maintenance
For recommendations on general instrument maintenance, refer to the Experion system manual
(bulletin 10001312).
Clean the electrodes after each run (routine cleaning). Cleaning maintains the instrument in optimum
condition and prevents buildup and cross-contamination of reagents and samples.
Perform the deep cleaning procedure described in Appendix B to clean the electrodes:
n
Prior to first use of the Experion electrophoresis station
n
Whenever contamination is suspected or visible (for example, salt deposits or other
precipitates) on the electrodes
n
Whenever a chip has been left in the electrophoresis station for an extended period of time
(for example, overnight)
Never store the cleaning chip inside the electrophoresis station. Store the empty cleaning chip covered
to keep the wells clean. A new cleaning chip is included with every box of chips.
2.5 Experion Video Tutorials
For additional information, view the video tutorials available online at www.bio-rad.com:
North America: Home > Life Science Research > Support > Tutorials > Electrophoresis and Blotting >
Experion System Training
Other: Home > Life Science Research > Electrophoresis > Automated Electrophoresis >
Experion Training Videos
Technical Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com 7
Experion Pro260 Analysis Kit