Experion automated electrophoresis system – Bio-Rad Experion Protein Analysis Kits User Manual
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of protein concentration, and the sample protein concentration is then determined from this
calibration curve and reported in the the Calib . Conc . column of the Results tab. Absolute
quantitation methods are often more accurate than relative concentration methods
How Experion Pro260 Analysis Differs from SDS-PAGE
Though the Experion Pro260 analysis kit and SDS-PAGE both use a gel-based matrix and voltage
difference to separate proteins according their size, there are a number of fundamental differences
between the two methods that may affect how specific proteins are separated and how data are
interpreted (see Table A1 below or refer to bulletins 5299, 5423, and 5453).
Table A.1. Comparison of Experion Pro260 analysis and SDS-PAGE.
Aspect
SDS-PAGE
Experion Pro260 Analysis
Sieving polymer (matrix)
Formulation can be changed to affect resolution
Fixed formulation
Crosslinked polymer
Polymer (not crosslinked)
Protein stain
Multiple available
Single, proprietary stain
Binds proteins
Binds LDS micelle-protein complexes
Detection
Separate staining step, proteins stained after
separation
Integrated separation step; proteins stained
during separation
Separate destaining step
Integrated destaining; proteins destained after
separation
Requires photography or other imaging
equipment for documentation, analysis
Laser excites the stain, detection using a
photodiode, both incorporated
Types of separation
Denaturing and native; reducing and
nonreducing conditions
Denaturing; reducing and nonreducing conditions
Downstream applications
Separated proteins available for excision,
electroelution, blotting applications
Proteins not available for further analysis
Reagent and sample usage
Moderate to extensive (ml to L)
Minimal (µl)
Waste generation
Moderate to extensive (ml to L)
Minimal (µl)
Overall analysis time
Hours (depends on gel size, staining method,
and methods of documentation and analysis)
30 min/chip (10 samples per chip)
Data analysis
Manual; requires additional equipment and
software
Automated (with flexibility of manual adjustments)
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Experion Automated Electrophoresis System