Bio-Rad Experion Protein Analysis Kits User Manual
Page 60

Table C.5. Chemicals with mild effects on Experion Pro260 separation or analysis.
Chemical
Concentration
Effect(s)
Acetone
15%
Quantitation affected
Bio-Lyte
®
3/10
0.75%
Quantitation affected
Guanidine HCl
25 mM
Enlarged system peak shifts to right; 10 kD sizing affected
KCl
1.5 M
Quantitation affected
MES
50 mM, pH 6.0
Quantitation affected
MOPS
100 mM, pH 7.2
Quantitation affected
PEG
0.50%
Quantitation affected
Propylene glycol
50%
Enlarged system peak shifts to right; 10 kD sizing affected
Sucrose
200 mM
Quantitation affected
Table C.6. Chemicals with severe effects on Experion Pro260 separation or analysis.
Chemical
Concentration
Effect(s)
Ammonium sulfate
1.5 M
Protein precipitation
Guanidine isothiocyanate
1 M
Protein precipitation; no markers appear
Guanidine HCl
1 M
Protein precipitation; no markers appear
HCl
150 mM
Protein precipitation; markers missing
Pluronic F-68
0.1%
Protein smears
Detergents and Reductants
Table C.7. Detergents and reductants with no observable effects on Experion Pro260 separation or analysis.
Detergent/Reductant
Concentration
ASB-14
0.10%
CHAPS
0.50%
CHAPSO
0.10%
Deoxycholate
1.0%
Octyl-thioglucopyranoside
0.50%
Tributylphosphine (TBP)
7.5 mM
Triton X-100
0.40%
Tween-20
0.50%
Table C.8. Detergents and reductants with mild effects on Experion Pro260 separation or analysis.
Detergent/Reductant
Concentration
Effect(s)
ASB-14
0.5%
Enlarged system peak shifts to right; 10 kD sizing affected
CHAPS
1%
Enlarged system peak shifts to right; 10 kD sizing affected
CHAPSO
0.2%
Enlarged system peak shifts to right; 10 kD sizing affected
Deoxycholate
2%
Enlarged system peak shifts to right; 10 kD sizing affected
LDS
3%
Enlarged system peak shifts to right; 10 kD sizing affected
NP40
0.5%
Enlarged system peak shifts to right; 10 kD sizing affected
SB3-10
0.1%
Enlarged system peak shifts to right; 10 kD sizing and quantitation
affected
SDS
1%
Quantitation affected
Tween-20
0.75%
Enlarged system peak shifts to right; 10 kD sizing affected
Zwittergent 3-14
0.10%
Enlarged system peak shifts to right; 10 kD sizing affected
β-Mercaptoethanol
1.5 M
Quantitation affected
DTT
150 mM
Quantitation affected
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Experion Automated Electrophoresis System