Bio-Rad T100™ Thermal Cycler - Manual (Page 26)

Skipping Steps in a Protocol
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Skipping Steps in a Protocol
Skip a step if it is necessary to shorten a protocol while it is still running. By repeatedly
skipping steps, it is possible to bypass several GOTO loops and shorten a protocol.
To skip the current protocol step, follow these steps:
1. View the current step from the Status screen.
2. Touch Skip Step to bypass the current step.
3. Touch Skip Step multiple times to skip more than one step.
Canceling a Run
A protocol may be canceled while it is running. When the protocol is canceled, the block
immediately stops changing temperature.
NOTE: Do not turn the thermal cycler off immediately after canceling a run. The
fans may need to run to cool the block.
To cancel a protocol:
1. Touch Cancel from the Status screen, and touch Yes to confirm.
Incubating Samples
To incubate samples at a constant temperature for any amount of time:
1. Touch Incubate from the home screen. The Incubate screen opens (Figure 20).
Figure 20. The Incubate screen.
NOTE: When incubation is occurring at temperatures below 30°C, the lid will
maintain a temperature of 31°C to prevent excessive condensation.
2. Edit the Block temperature, Lid temperature, and Hold time parameters.
3. Touch Run to start the incubation.
4. To end the incubation, select Cancel.
WARNING! Incubating samples for extended periods of time at 4–10°C,
particularly in areas of high humidity, can cause excessive moisture condensation
around the block.