Bio-Rad T100™ Thermal Cycler - Manual (Page 16)

Creating a New Protocol or Editing an Existing Protocol
6
.
Figure 6. The template for a PCR protocol.
4. The protocol editor displays the protocol in a graphical format using editable boxes.
5. To edit the temperature or time for each step, the sample volume, and the lid
temperature, touch the appropriate box and enter a new value in the numeric keypad.
6. Touch Run to run the protocol immediately without saving it. Alternatively, touch Save to
choose a folder location and save the protocol, and then touch Run to run it (page 13).
Protocol Parameters
Table 2 includes a list of all the parameters for temperature and gradient steps.
Table 2. Protocol steps and parameters of the T100™ thermal cycler.
Step Name Parameters and Ranges Description
Temperature Temperature in °C : The target
temperature between 4.0 and
100.0°C in tenths of a degree
Instructs the thermal cycler to ramp to
the target temperature and hold that
temperature for a specified time
Time The hold time between 1 sec
and 18 hr in the format of
hr:min:sec. To enter an infinite
hold, touch the
∝ (infinity, 0) key
Gradient Front row: The lower
temperature in the gradient.
Enter a number between 30.0
and 99.0°C in tenths of a degree
Back row: The upper
temperature in the gradient. The
maximum temperature is 100°C.
Enter a temperature within
25.0°C of the lower temperature
Instructs the thermal cycler to ramp to
the target temperature gradient across
the block and hold that temperature
gradient for a specified time