Bio-Rad T100™ Thermal Cycler - Manual (Page 18)

Creating a New Protocol or Editing an Existing Protocol
8
Inserting a Protocol Step
Insert a protocol step if a new temperature, GOTO, or gradient step is needed:
1. Select a step to the left of where the new step is to be inserted.
2. Touch Insert. A pop-up menu displays types of steps to insert (Figure 8).
Figure 8. Insert a new step to the right of step 1.
3. Touch Temperature to insert a temperature step, Gradient to insert a gradient step, or
GOTO to insert a GOTO step to create a cycle for PCR.
4. Edit the parameters in the new step as desired.
Deleting a Protocol Step
To remove a step from a protocol:
1. Select the step to be deleted.
2. Touch Delete to remove the selected step.
Adding or Removing a Temperature Gradient
To add a temperature gradient to a step:
1. Select a step and touch Options to open the Step Options window (Figure 9).
Figure 9. The Step Options window.