Bio-Rad T100™ Thermal Cycler - Manual (Page 20)

Creating a New Protocol or Editing an Existing Protocol
10
Temperature Increment, Extend, and Ramp Rate
To add increments, extensions, and ramp rates to a step:
1. Select a step and touch Options to open the Step Options window (Figure 12).
2. A step can contain all three options. To edit an option, touch the field for Increment,
Extend, or Ramp rate.
3. Enter a value using the numeric keypad, and touch OK.
Figure 12. Step 4 has a temperature increment option.
To remove the increment, extension, or ramp rate,
1. From the Step Options window, touch the field for the option and change the value to
zero using the numeric keypad.
2. Touch OK to confirm.
Changing Parameters in a GOTO Step
The GOTO step instructs the thermal cycler to repeat a set of steps in a cycle. To edit the
GOTO step:
1. Select a GOTO step.
Figure 13. A protocol that includes a GOTO step 2 and 34 additional repeats.
2. To change the step that the cycler will start repeating, touch the Step field.