Bio-Rad T100™ Thermal Cycler - Manual (Page 25)

T100 Thermal Cycler Manual
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Figure 18. The Clock screen.
Pausing and Resuming a Run
A running protocol can be paused temporarily. During a pause, the thermal cycler maintains
the block temperature of the current step and the lid temperature until the run is resumed. If it
is paused during ramping, then it will continue ramping until it reaches the target temperature
of the current step.
TIP: After a power failure, the T100™ thermal cycler automatically resumes a run
and displays a warning message when the cycler turns back on.
To pause and then resume a running protocol, follow these steps:
1. Touch Pause from the Status screen.
2. To resume the protocol, touch Resume (Figure 19).
WARNING! Pausing a step can adversely change the outcome of the experiment.
If the protocol is paused during a temperature step, the reactions are held at the
target temperature for longer than the protocol requires.
Figure 19. The running protocol is paused at step 4.