1 introduction to the t100™ thermal cycler, System overview, Introduction to the t100™ thermal cycler – Bio-Rad T100™ Thermal Cycler User Manual
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T100 Thermal Cycler Manual
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Introduction to the T100™ Thermal
Cycler
Read this chapter for information on setting up the T100 thermal cycler.
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System overview (page 1)
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Setting up the T100 thermal cycler (page
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Selecting compatible reaction vessels and sealing options (page
System Overview
The T100 thermal cycler has a 96-well block that is compatible with standard height tubes,
tube strips, and plates. It can run sample volumes of 1–100 µL, with volumes of 15–100 uL
recommended for the best reproducibility. It is composed of the following parts:
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Reaction block — holds the plates, tubes or tube strips containing samples
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Heated inner lid — heats the top of the samples to prevent evaporation and
condensation
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Touch screen — provides the means to control the cycler with gloved or ungloved
hands
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USB port — connects to a USB flash drive for data transfer
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Status LED — turns on during normal operation; blinks when the cycler is in standby
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Air vents — provide ventilation to allow the thermal cycler to heat and cool quickly