Bio-Rad T100™ Thermal Cycler - Manual (Page 14)

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2. Load the samples
To ensure uniform heating and cooling of samples, vessels must be in complete contact with
the reaction block. Adequate contact is achieved by:
• Confirming that the block is clean before loading samples
• Firmly pressing the individual tubes or the microplate into the block wells
NOTE: When running individual tubes, place the support ring in the block to
prevent deformation of the tubes by the heated lid. The ring is reversible for domed
cap tubes or flat cap tubes (Figure
4). Alternatively, load eight empty tubes in the
corners and sides of the block.
3. To close the lid, push the lid handle down until it stops.
Figure 4. Tube support ring for running a few individual tubes.
Compatible Reaction Vessels and Sealing Options
The T100 thermal cycler is compatible with full-height strips, tubes, and plates. Bio-Rad tests
the standard supplies listed in Table
1 for compatibility with the T100 thermal cycler.
The composition and thickness of reaction vessels influence the outcome of a reaction.
Microplates, tubes, sealers, and caps come in a variety of compositions and colors. For a full
list of available reagents and supplies, refer to the Life Science Research product catalog or
the website at discover.bio-rad.com.
* The prefix of the catalog number for each series of supplies.
Table 1. Compatible microplates, tubes, and sealing options for the T100 thermal cycler.
Tubes and Microplates Caps and Sealers
Description
(Catalog Prefix*)
Volume and Number
Description
(Catalog Prefix*)
Tubes Caps (TCS*)
Individual tubes with caps (TFI* and TWI*) 0.2 ml (Included)
Strip tubes (TBS*) 0.2 ml, 8- and 12-tube Strip
Microplates Film (MS*) Caps (TCS*)
Unskirted and full-height (MLP*) 96-well A, B, F Strip
iQ full height plates (223-9441) 96-well A, B, F Strip
Semi-skirted, full-height, and hard-shell
(HSS*) 96- well A, B, F Strip