Bio-Rad T100™ Thermal Cycler - Manual (Page 19)

T100 Thermal Cycler Manual
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2. Select the Gradient checkbox. The temperatures for each row are displayed (Figure 10).
3. Touch the Back Row and Front Row fields to edit the gradient, and touch OK.
NOTE: A gradient range must be between 1 and 25°C.
NOTE: A gradient step can also include an Extend parameter, but the Increment
and Ramp Rate options are not allowed.
Figure 10. The Step Options window displays the gradient temperatures.
4. The front and back row temperatures of the gradient are displayed as two lines in the
thermal profile (Figure 11).
5. The gradient can be edited by touching the temperatures directly without opening the
Step Options screen.
Figure 11. The protocol has a gradient from 62 to 72°C.
To remove the temperature gradient:
1. Select the gradient step and touch Options to open the Step Options window.
2. Deselect the Gradient checkbox.
3. Select OK to confirm.
Back and front
row temperatures