Bio-Rad T100™ Thermal Cycler - Manual (Page 17)

T100 Thermal Cycler Manual
7
Changing Target Temperature and Hold Time
To change the target temperature and hold time in a temperature step:
1. From the protocol editor, touch the temperature or hold time for a step.
2. A pop-up keypad appears to allow you to to edit the value (Figure 7).
3. Enter a new value and touch OK.
Figure 7. Editing a temperature with the numeric keypad.
Increment A temperature from –10.0 to
10.0°C per cycle in tenths of a
degree Applies only to a temperature step.
Instructs the thermal cycler to
increment (change) the target
temperature of a step with each cycle,
where a positive number increases the
temperature and a negative number
decreases the temperature
Ramp Rate A number from 0.1 to 4°C per
sec Applies only to a temperature step.
Instructs the thermal cycler to ramp to
the target temperature at the specified
ramp rate in that step. If not specified,
the T100 thermal cycler runs at a
maximum ramp rate of 4°C/sec
Extend A time from –60 to 60 sec per
cycle Applies to both temperature and
gradient steps. Instructs the thermal
cycler to extend the hold time with
each cycle. A positive number
increases the hold time and a negative
number decreases the hold time.
Table 2. Protocol steps and parameters of the T100™ thermal cycler. (continued)
Step Name Parameters and Ranges Description