Sample volume and lid temperature, Temperature control modes, Changing sample volume – Bio-Rad T100™ Thermal Cycler User Manual
Page 21: Changing lid temperature
T100 Thermal Cycler Manual
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3. Enter a value for the first step in the GOTO loop using the numeric keypad and touch OK.
4. To change the number of times the cycler will repeat the cycle, touch the X field.
5. Edit the number of repeats using the numeric keypad and touch OK.
NOTE: The X field is the number of additional repeats. For example, 34 X will repeat
steps 2–4 an additional 34 times for a total of 35 cycles of PCR.
NOTE: A protocol may contain multiple GOTO loops but they may not be nested
with one loop inside another.
Sample Volume and Lid Temperature
•
Sample volume — determines the temperature control mode, which influences the
amount of time the samples are held at the target temperature
•
Lid temperature — determines the temperature of the heated lid
The T100 thermal cycler provides three ways to enter sample volume and lid temperature:
•
Change the setting when creating and editing the protocol (page
•
Override the sample volume setting while initiating a run (page
•
Set a default for the sample volume for new protocols in Settings (page
Temperature Control Modes
The T100 thermal cycler uses one of two temperature control modes to determine when the
sample reaches the target temperature:
•
Calculated mode — the thermal cycler calculates the sample temperature based on
the sample volume when a sample volume between 1 and 100 µl is entered.
•
Block mode — when a sample volume of zero (0) µl is entered, the thermal cycler
assumes that the sample temperature is the same as the measured block
temperature
Changing Sample Volume
To change the sample volume for a protocol:
1. From the New Protocol screen, touch the Volume field.
2. Using the numeric keypad, enter the sample volume and touch OK.
3. Entering a sample volume between 1 and 100 µl selects Calculated mode. Entering a
sample volume of zero (0) selects Block mode. Calculated mode is the recommended
mode because it most accurately represents the actual sample temperature.
Changing Lid Temperature
Heating the lid prevents condensation from forming inside the reaction wells. Without a heated
lid, water can be lost to condensation, concentrating the reactants in the tube or plate.
The T100 thermal cycler lid can be programmed to heat between 40 and 110°C. The default
recommended lid temperature is 105°C.
NOTE: When the block is running an infinite hold at a temperature below the cutoff
temperature of 30.0°C, the lid heater maintains 31.0°C.