Bio-Rad CFX Automation System II - Manual (Page 34)

CFX Automation System II 26 |
Chapter 2: Using the CFX Automation System II
The CFX System instruction manual (bulletin #10021337) explains in detail the steps required to
set up LIMS integration. These steps include:
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Setting up a LIMS folder and data export options
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Creating a LIMS file (*.plrn) using the provided CFX96 ™ or CFX384 ™ LIMS Plate Import
Template files. Detailed definition of the LIMS Plate Import Template file content is provided
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Exporting data as a LIMS file
To complete LIMS integration, CFX Manager Software requires plate setup information
generated by the LIMS platform, a protocol file created using CFX Manager Software (*.prcl), a
defined data export location, and a defined export format. The LIMS file created by your internal
LIMS contains the plate setup details and the protocol file name. CFX Manager Software will
use the LIMS file to create a plate file that will be used in conjunction with the named protocol
file to start a run and generate data. Refer to the LIMS Integration Section in the CFX System
instruction manual (bulletin #10021337) for more detail.
When LIMS files are created for use with the automation system, plate barcode information
provided in column B, row 8 of the LIMS file (the Run ID field) can be directly imported into the
automation software (see Entering Barcode Information into a Plate List). Information entered in
column B, row 11 of the LIMS file (the Data File field) can be used to name data files, reports,
and export files (see File Name Format).
Once LIMS integration is complete, LIMS files may be loaded into the automation software and
LIMS data export files can be generated.
PrimePCR Information
Bio-Rad offers 96- and 384-well PrimePCR plates that contain primer pairs lyophilized in
the plate wells. You may customize the genes on the plates or choose from a wide variety of
predesigned disease- and pathway-specific panels. For more information, go to
www.bio-rad.com/primepcr.
Bio-Rad provides run files containing gene layout and cycling protocol information for all
PrimePCR plates. Run files for predesigned and custom plates can be downloaded from
www.bio-rad.com/primepcr. Run files for custom plates are saved under My PrimePCR
once configured and purchased. To obtain run files for predesigned panels, select Run File on
the Review Plate webpage.
Once the run files are downloaded, easily load PrimePCR files into any automation plate list
using the plate list controls.
Create the Plate List: Additional Features
Quickly Add Rows to the Plate List
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Add multiple rows with the same protocol and plate files (or same LIMS or PrimePCR
files). Enter the desired number of plates in the Rows box before selecting Add
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Apply identical protocol and plate files (or LIMS or PrimePCR files) to all plates in a
rack. Enter the protocol and plate file (or LIMS or PrimePCR file) into a single row and select
Repeat row 1 until rack is empty. The protocol and plate files will be applied to all plates
in the input rack. Each time a new plate is retrieved from the source rack, a new row will be
automatically added to the plate list. This option is available only when a single row is defined
in the plate list