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Starting an automation run – Bio-Rad CFX Automation System II User Manual

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CFX Automation System II

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Chapter 2: Using the CFX Automation System II

Open the CFX Automation Control Software by double clicking the automation system icon. The
main software tabs in the CFX Automation Control Software include:

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Status tab. Illustrates the status of the plate lists, the configured CFX System(s), and the
waste rack

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Plate list tabs. Named according to the rack on which the source plates are loaded

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History tab. Provides a log of plates run using the CFX Automation System II

Starting an Automation Run

The plate list is the table that contains the cycling instructions for all plates loaded into a specific
source rack (Figure 19). The plate list tabs are named according to the rack in which the source
plates are loaded (Rack 1, Rack 2, etc). View each of the plate lists by selecting either of the
plate list tabs on the main tool bar. All controls for creating, editing, saving, and running plate
lists are found on the plate list window.

Basic automation runs can be started by following these five steps:
1. Create the plate list.
2. Select the CFX System(s).
3. Modify the barcode controls.
4. Load the plates.
5. Select Start.

Fig. 19. Plate list tab.

Select barcode
cell to be
modified

Plate list
controls

Status tab

Plate list tabs

History tab

CFX System selection

Barcode
controls

Import LIMS or
PrimePCR run
ID as barcode

Selection box
to highlight an
entire row