1 introduction, 1 instrument description – Metrohm 817 Bioscan User Manual
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1.1 Instrument description
817 Bioscan
1
1 Introduction
1.1 Instrument
description
The 2.817.0010 Bioscan is a PC-controlled measuring instrument for
the sensitive analysis of carbohydrates by ion chromatography using
pulsed amperometric detection. The compact housing of the 817 Bio-
scan contains several IC system components:
• Detector – flow-through cell with three-electrode arrangement for
amperometric detection in the Pulse, DC and Scan modes.
• Column compartment – the perfect insulation of the housing cre-
ates not only stable thermal conditions for the separating columns,
but also shields the system from electromagnetic interference; in
addition to the column it also contains the detection cell, pulsation
absorber and preheating capillary.
• Oven – amperometric determinations require extremely stable
thermal conditions. The built-in column compartment oven ensures
that all important components can be set exactly to a temperature
from 10°C above room temperature to 60 °C with a stability of
0.1°C.
• All components that come into contact with the eluent and the
sample, except the electrodes, are metal-free.
• Signal converter – the 817 Bioscan contains its own analog/ digi-
tal converter for the detector signal. This means that complete con-
trol of the instrument is possible from a PC via an RS232 interface.
Together with the 709 IC Pump and the 812 Valve Unit, the 817 Bio-
scan forms a complete IC system for carbohydrate analysis. Opera-
tion is via a PC connected to the RS232 interface and uses the in-
cluded
«IC Net 2.1» control and evaluation program. This software
fulfills all the demands which are placed today on a modern integration
software: 1-point or multi-point calibration, internal or external standard,
selectable algorithms for non-linear calibration, numerous Integration
modes with selectable parameters and integration events, various peak
recognition methods, peak editor, free scaling, superimposition of sev-
eral chromatograms, post-treatment of chromatograms, high-perform-
ance GLP-conform report generator with output interfaces for monitor,
printer and external databases.