3 connection of the injection valve, 4 mounting the column – Metrohm 817 Bioscan User Manual
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2 Installation
817 Bioscan
16
2.3.3
Connection of the injection valve
Connect all capillaries to the injection valve according to the following
description:
2
1
4
5
3
6
42
43
44
47
46
45
Fig. 5: Connections at the injection valve of 2.812.0010 valve unit
42
Connection for syringe tubing
PTFE tubing with coupling
(6.2744.120) and syringe (6.2816.020,
without needle)
45
Eluent outlet
Connection to preheating capillary
31
(PEEK, 6.1836.010)
43
Connection for aspirating tubing
PTFE tubing for aspirating sample
46
Eluent inlet
PEEK capillary connection
29 to
pulsation absorber
44
Connection for sample loop
20 µL PEEK sample loop (6.1825.210)
47
Connection for sample loop
20 µL PEEK sample loop (6.1825.210)
2.3.4
Mounting the column
The IC separating column (e.g. Metrosep Carb 1, 6.1013.000) is first
mounted in the 817 Bioscan as follows (see Fig. 3):
1 Connect column to the preheating capillary
• Remove the caps from column 25.
• Screw eluent outlet of preheating capillary 31 on to the inlet
end of the column (observe flow direction) .
2 Connect column to flow-through measuring cell
• Screw outlet end of column 25 on to eluent inlet 41 of the
flow-through cell using the 6.2744.130 KELF pressure screw.
3 Fix column
• Fix the column in column holder 26.