Metrohm 817 Bioscan - Manual (Page 70)

5 Notes – Maintenance – Faults
817 Bioscan
62
Signal "overload" • Wrong IC-Net parameter
' Range ' and ' Fullsca-
le '
• Damaged reference
electrode
• Damaged working
electrode
• Flow cell is not con-
nected properly
• Wrong measuring
potential
• Check settings
• Check and replace
reference electrode
• Replace working electrode
• Check cable connections
(REF: black, WE: red,
AUX: blue)
• Optimize measuring
potential
Considerable
pressure drop
• Leak in system • Check connections and
make leakproof
Considerable
pressure rise
• Contamination of the
filter in the 6.2821.100
Filter unit PEEK
• Change of column
packing by injection of
contaminated samples
• Mounting screws 36 of
flow cell overtighten;
25 µm spacer has been
used
• Replace the 6.2821.110
Filter
• Regenerate the column
(see section 5.1.1) or
replace column
Note:
Samples should always
be microfiltered.
• Check screws of flowcell;
use 50 µm spacer
Chromatograms
with poor resolu-
tion, change in the
retention times • Deterioration in
separation efficiency of
the IC column
• Old Eluent
• Ionic strength or pH of
the sample differs
strongly from eluent
• Regenerate the column
(see section 5.1.1) or
replace column
• Replace eluent
• Dilute sample or change
pH of sample
Extreme peak
broadening,
splitting (double
peaks) • Dead volume at the
column ends
• Dead volume in IC
system
• Replace column
• Check connections
No signal from
detector • No mains current
• 817 Bioscan not
switched on
• Faulty fuse
• Incorrect mains voltage
• Measuring cell not
connected
• Dirty working electrode
• Connection to IC Net is
not working properly
• Check mains supply and
voltage (see Section 2.2)
• Switch on the 817 at the
rear panel
• Replace fuse (see section
2.2.1)
• Check mains voltage (see
Section 2.2.1)
• Check connection
• Clean working electrode
(see Section 5.2.3)
• Check connection