Metrohm 817 Bioscan - Manual (Page 23)

2.3 Capillary connections
817 Bioscan
15
2.3 Capillary connections
The capillary connections described below are necessary for operating
the 817 Bioscan in a simple IC system for carbohydrate analysis (MIC-
8). The electronic connections and control by IC Net 2.1 are described
in Section 2.4.
Section 2.5 contains the description of a combination of this IC system
with an IC system for the determination of anions with chemical sup-
pression (MIC-9). The necessary changes to the capillary connections
are described there.
2.3.1 Connection of the 6.5324.000 Bottle rack (option)
The optionally available 6.5324.000 Bottle rack for supply vessels can
be placed on top of the 817 Bioscan. The tubing connections to the 709
IC Pump are described in the corresponding leaflet.
2.3.2 Connecting the pulsation absorber
To protect the column material from any pressure shocks which the in-
jection may cause we recommend the insertion of the 6.2620.150 MF
Pulsation absorber between the high-pressure pump and the injec-
tion valve. This is done as follows (see Fig. 3):
1 Connect the pulsation absorber
• Place pulsation absorber 30 on the floor in the interior of the
817 Bioscan.
2 Connection to pump
• Connect the 6.2821.100 PEEK Filter unit described in
Section 2.3.5 of the 8.709.1021 ‘Instructions for Use’ to con-
nection
23 of the 709 Pump by using PEEK capillary 22.
• Lead PEEK capillary 28 or 43 from connection piece 47 of
the filter unit outwards through opening 2 of the Bioscan and
attach it to one of the 52 connections on the top side of the
pulsation absorber.
3 Connection to injection valve
• Connect PEEK capillary 29 to the second connection 30 of
the pulsation absorber, lead it out through 2 of the Bioscan
and connection it to the injection valve on the 812 Valve Unit
(connection
17).
The pulsation absorber is filled with isopropanol and must be rinsed
with eluent before a separating column is connected (see Section
2.3.7).
The 6.2620.150 Pulsation absorber can be operated in both direc-
tions.