Metrohm 817 Bioscan - Manual (Page 33)

2.5 System for the analysis of anions and sugars – MIC-9
817 Bioscan
25
2.5.2 Capillary connections
This section describes the capillary connections required for the com-
bination of the IC system for sugar analysis (MIC-8) with an anion sys-
tem with chemical suppression (e.g. MIC-2). It is assumed that the cap-
illary connections for the anion system are already known.
Arrange the necessary components as shown in Fig. 9. Please also ob-
serve the recommendations about the arrangement of the IC modules
given in Section 2.1.4. The capillary connections for the 817 Bioscan
correspond to a large extent to the connections described in Section
2.3. Only the connection of the injection valve to the 812 Valve Unit is
not necessary. Instead the second injection valve B of the 733 IC Sepa-
ration Center is connected for injecting the sample for the sugar deter-
mination (see below).
For the components for determination of the anions the typical ar-
rangement of the capillaries for such an anion system is assumed to be
present. The necessary alterations concern the injection valve A in the
733 IC Separation Center, which must be connected for injection valve
B so that the two sample loops can be filled simultaneously. In addition
the MSM Suppressor Module connections must be transferred to the
753 IC Suppressor Module if the 752 IC Pump Unit has previously been
used with the MSM Module contained in the 733 IC Separation Center.
The necessary modifications are described below. Injections valves A
and B are designated as follows:
Injection valve A: sample injection for anion determination
Injection valve B: sample injection for sugar determination
All the connections for the two injection valves are shown in Figure 10
on the following page.
1 Connection injection valve A – injection valve B
For simultaneously filling the two sample loops as short as
possible piece of PEEK capillary is attached to sample outlet
48
of injection valve A and sample inlet 55 of injection valve B.
2 Connection pulsation absorber – injection valve B
Connect PEEK capillary 29 to pulsation absorber 52 in the
Bioscan, lead it out through opening 2 or 3 of the Bioscan, and
in through opening 27 or 28 of the 733 IC Separation Center
and connect it to injection valve B (eluent inlet 58).
3 Connection injection valve B – preheating capillary
Connect PEEK capillary at injection valve B (eluent outlet 57),
lead it out through opening 27 or 28 of the 733 IC Separation
Center and in through opening 2 or 3 of the Bioscan and use a
PEEK coupling to connect it to preheating capillary 31.