Leica Biosystems LN22 - Manual (Page 4)

4 Instructions for Use V1.2, RevB – 10/2012
Table of contents
1. Important information ................................................................................................................ 6
1.1 Symbols in the text and their meanings ................................................................................... 6
1.2 Intended use of instrument ........................................................................................................ 6
2. Safety ............................................................................................................................................ 7
2.1 Safety instructions ...................................................................................................................... 7
3. Instrument components and specifications ....................................................................... 15
3.1 Technical data ............................................................................................................................ 15
3.2 Overview and instrument components .................................................................................. 16
3.3 Standard delivery ...................................................................................................................... 17
4. Startup ........................................................................................................................................ 18
4.1 Installation site requirements .................................................................................................. 18
4.2 Unpacking ................................................................................................................................... 18
4.3 Preparing the microtome for attachment of the LN22 ......................................................... 19
4.4 Installing the cryochamber ...................................................................................................... 20
4.5 Connecting to mains ................................................................................................................. 21
4.5.1 Checking the voltage selector setting ................................................................................... 21
4.5.2 Changing the voltage selector setting and inserting the fuses ......................................... 22
4.5.3 Plugging in the connection cables ......................................................................................... 23
4.5.4 Grounding the Dewar ................................................................................................................ 23
4.5.5 Connecting the mains cable .................................................................................................... 24
4.6
Switching on the instrument .................................................................................................... 24
4.7 Control unit ................................................................................................................................. 25
4.8 Specimen holders ...................................................................................................................... 27
4.8.1 Specimen holder for block embeddings ................................................................................ 27
4.8.2 Universal specimen holder and flat specimen holder ......................................................... 29
4.9
Knife holders .............................................................................................................................. 30
4.9.1 Knife holders for tungsten carbide knives ............................................................................. 30
4.9.2 Glass knife, CryoDiamond knife, trimming knife Histo ......................................................... 30
4.10
Preparing the pumping system ............................................................................................... 33
4.11 Refrigerating .............................................................................................................................. 33
4.12 Refilling the Dewar vessel ....................................................................................................... 34
4.13 Heating up the chamber ........................................................................................................... 35