Leica Biosystems LN22 - Manual (Page 32)

32 Instructions for Use V1.2, RevB – 10/2012
4. Startup
Fig. 26
Adjusting the knife holder laterally:
• Release the clamping screws (10, Fig. 24) of the knife holder base
(9, Fig. 24).
• Displace the knife holder laterally via the eccentric disc (12, Fig. 24).
Changing the knives inside the cryochamber
• Prior to changing knives, move the cryo specimen arm (1, Fig. 24) first
to the rear limit stop and next to the lower limit position.
• Lock the handwheel.
• Using special forceps (Fig. 26) knives can be exchanged inside the
cryochamber at very low temperatures.
4.9.2 Glass knife, CryoDiamond knife, trimming knife Histo
Fig. 27
• Insert the cryo-diamond (8) or glass knife in the knife holder (9); tighten
clamping screws (10).
• See ‘Knife holder for tungsten carbide knives’ on how to proceed.
The trimming knife kit can be used to trim lateral specimen edges parallel
before sectioning with glass or diamond knives.
• It is possible to exchanges the knives inside the cryochamber at very
low temperatures using the standard forceps. Prior to exchanging
the knives, displace the cryo specimen arm to the rear limit stop.
• Lock the handwheel.
Fig. 28
10
10
9
8
Manipulator