Leica Biosystems LN22 - Manual (Page 16)

16 Instructions for Use V1.2, RevB – 10/2012
3.2 Overview and instrument components
3. Instrument components and specifications
1
2
5
3
4
1 Cryochamber
2 Control unit
3 Liquid nitrogen
pump
4 Dewar vessel for
liquid nitrogen on
roller base
5 Connection cable
6 LN
2
connection
hose
The liquid nitrogen freezing device LN22 quickly
converts a rotary microtome into a cryosectioning
system.
The LN22 attachment consists of a cryochamber,
which encloses the sectioning area, and which
is refrigerated by a permanent flow of liquid ni-
trogen, pumped from a Dewar vessel. The auto-
matic pumping mechanism ensures a stable low-
temperature environment around the frozen
specimen and the knife. Separate temperature
control systems maintain stable temperatures of
both knife and specimen.
The desired temperature setting for knife and
specimen are selected on the control unit. The
control unit, which continuously monitors and
regulates the selected temperature parameters
and controls the liquid nitrogen pumping system.
Actual temperatures of knife, specimen and
cryochamber are displayed on the control unit at
all times.
The cryochamber is mounted onto the microtome
base plate, thus being completely isolated from
the microtome feed and drive elements.
Fig. 9
6