Leica Biosystems LN22 - Manual (Page 29)

29 Leica LN22
4.8.2 Universal specimen holder and flat specimen holder
• Place the specimen into the specimen holder
(5) and (6) slightly tighten screw (Use special
tool!)
• Use the special forceps with large radius to
insert the specimen holder into the cryo speci-
men arm and tighten loosely with screw (3) of
cold bridge (4).
Fig. 23
6
5
• Release handwheel lock.
• Place the specimen cylinder into the lowest possible position to en-
sure a fast freezing process.
• Once the desired temperature has been reached, bring the specimen
cylinder back in the upper limit position.
• Lock the handwheel.
• Tighten screw (6) in specimen holder (5).
• Loosen screw (3, Fig. 22) of cold bridge (4, Fig. 22) from the cryo
specimen arm, orient the specimen holder into the desired cutting
direction.
• Tighten screw (3) or cold bridge (4) thoroughly.
Extra Fine Screw Thread! Do not tighten too much!
4. Startup