Leica Biosystems LN22 - Manual (Page 10)

10 Instructions for Use V1.2, RevB – 10/2012
• There are clothing items and protective gear
with certain characteristics which you should
specifically avoid when working with LN
2
: Do
not wear closed safety goggles (a), boots (c),
shoes (e) and gloves (g) into which LN
2
splashes can enter, but from where they can-
not escape. LN
2
that has splashed into such
items will evaporate immediately inside the
item thus causing severe injuries, before the
item can be removed or taken off. Therefore,
always use safety goggles with side protec-
tion but which are open at the top and at the
bottom (b), so that LN
2
splashes can escape
immediately. Wear boots only in combination
with long, loose-fitting (not tight!) pants that
fall over the boots (d) and completely cover
their upper opening. Only wear open slippers
(f) in the lab, no shoes or pumps that enclose
your feet entirely.
In combination with open slippers/sandals only
wear pants without cuffs, so that LN
2
splashes
will flow off freely. When pouring LN
2
from one
container into another or when inserting the
Dewar pump into the Dewar, never wear safety
gloves! As an alternative to gloves you can use
an open flannel cloth (h) to protect your hands
when touching cold components. Protective
gloves are useful when handling cold and dry
objects, however they are not for work with LN
2
.
a
c
b
d
f e
g h
• If your skin is injured by splashing LN
2
, imme-
diately rinse the affected skin area(s) thor-
oughly with lukewarm (body temperature!)
water. In case of serious injuries, immediately
see a dermatologist.
If LN
2
splashes into your eyes, rinse the
eye immediately and thoroughly with
lukewarm water. If available in your lab,
use a special eye-rinsing device for that
purpose. After rinsing, immediately see
an ophthalmologist.
Fig. 1
2. Safety