Leica Biosystems SP2600 - Manual (Page 40)

40 Supplementary instruction manual V 1.2 – 07/2002
5.8 Finishing milling
• Attach the miller holder containing the finishing miller to the milling
spindle (Allen screws / Allen key size 5) - see Fig. 5.21, page 34).
Always slide the plexiglass cover to the upper limit position prior
to attaching the miller holders to the milling spindle!
• Bring the cutting edge of the miller close to the specimen surface and
thoroughly check the distance between miller cutting edge and speci-
men surface as described for the pre-miller on page 35.
• If necessary, adjust the milling window (button (61) and others - see
chapter 5.4.14, page 37 of Leica SM2500 instruction manual).
Setting the milling parameters for the finishing milling procedure
• Once again activate automatic mode of operation ‘AUTO’ and set the
milling parameters using the same control unit buttons as described for
the pre-miller on page 36.
Individual parameter settings depend on specimen material and
size. Please refer to the recommendations below and on the fol-
lowing page.
Recommended milling thickness settings for finishing milling
• 5 µm - keep milling until surface is completely level.
• 3 µm - three entire sledge strokes (milling and return stroke).
• 2 µm - three entire sledge strokes (milling and return stroke).
• 1 µm - three entire sledge strokes (milling and return stroke).
Do not exceed a milling thickness setting of 5 µm with the follow-
ing materials: brittle, very ductile materials such as titanium or fi-
berglass and compound samples containing such materials (such
as printed circuit boards).
5. Operation