7 pre-milling, Pre-milling, Operation – Leica Biosystems SP2600 User Manual
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Supplementary instruction manual V 1.2 07/2002
5.7 Pre-milling
Select automatic mode of operation AUTO (button (5) - chapter 5.4.3, page
26 Leica SM 2500 instruction manual).
In AUTO mode, set the milling parameters for the pre-milling procedure:
Select STOP or PHOTO position (buttons (23) or (26) chapter 5.4.8, page
31 - Leica SM2500 instruction manual).
Select milling thickness (button (10) / rotary selector switch (9) - see
chapters 5.4.4 and 5.4.5, pages 27/28 - Leica SM2500 instruction manual).
(--> see following page for recommended settings).
Activate retraction (button (10) / rotary selector switch (9) - see chapters
5.4.4 and 5.4.5, pages 27/28 - Leica SM2500 instruction manual).
Select milling and return stroke speed.
(button (19) / rotary selector switch (9) - see chapter 5.4.4, page 27 and
chapter 5.4.7, page 30 - Leica SM2500 instruction manual).
(see next page for recommended settings).
Close plexiglass protective cover.
Start miller rotation (button (37) - chapter 5.4.11, page 34 - Leica SM2500
instruction manual).
Select rotational speed of miller (buttons (39), (40) - chapter 5.4.11, page
34 - Leica SM2500 instruction manual).
Select desired milling mode (button (15) - chapter 5.4.6. page 29 - Leica
SM2500 instruction manual).
Individual parameter settings depend on specimen material and
size. Please refer to the following page for a list of recommended
settings.
5.
Operation
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