beautypg.com

7 pre-milling, Pre-milling, Operation – Leica Biosystems SP2600 User Manual

Page 36

background image

36

Supplementary instruction manual V 1.2 – 07/2002

5.7 Pre-milling

• Select automatic mode of operation ‘AUTO’ (button (5) - chapter 5.4.3, page

26 Leica SM 2500 instruction manual).

In ‘AUTO’ mode, set the milling parameters for the pre-milling procedure:
• Select STOP or PHOTO position (buttons (23) or (26) chapter 5.4.8, page

31 - Leica SM2500 instruction manual).

• Select milling thickness (button (10) / rotary selector switch (9) - see

chapters 5.4.4 and 5.4.5, pages 27/28 - Leica SM2500 instruction manual).

(--> see following page for recommended settings).

• Activate retraction (button (10) / rotary selector switch (9) - see chapters

5.4.4 and 5.4.5, pages 27/28 - Leica SM2500 instruction manual).

• Select milling and return stroke speed.

(button (19) / rotary selector switch (9) - see chapter 5.4.4, page 27 and

chapter 5.4.7, page 30 - Leica SM2500 instruction manual).
(see next page for recommended settings).

• Close plexiglass protective cover.
• Start miller rotation (button (37) - chapter 5.4.11, page 34 - Leica SM2500

instruction manual).

• Select rotational speed of miller (buttons (39), (40) - chapter 5.4.11, page

34 - Leica SM2500 instruction manual).

• Select desired milling mode (button (15) - chapter 5.4.6. page 29 - Leica

SM2500 instruction manual).

Individual parameter settings depend on specimen material and

size. Please refer to the following page for a list of recommended

settings.

5.

Operation

or

+

+

or