Eppendorf Research plus - Manual (Page 15)

5 Maintenance
15
Eppendorf Research® plus — Operating manual
5.2 Sterilizing or disinfecting the pipette
5.2.1 Autoclaving
All Research plus pipettes are completely steam autoclavable.
Before autoclaving
1. Remove any contamination from the exterior and in the lower part (see Research plus
disassembly and assembly on p. 17).
2. If you remove existing grease, slightly relubricate the piston seal using only the grease
specified in the ordering information (see the enclosed CD).
Procedure
Follow the operating manual of the autoclave manufacturer.
1. Autoclave at: 121°C; 20 minutes; 1 bar overpressure
2. When placing the Research plus into the autoclave, make sure that the temperature at the
pipette does not exceed 121°C.
3. You can put the Research plus into the autoclave as a whole unit or with the lower part
removed. Do not disassemble the lower part.
4. For 5 mL and 10 mL pipettes: remove the old protection filter. Add a new protection filter and
install it after autoclaving. Autoclave the protection filter only once.
5. If lower parts have been removed, make sure that no lower parts are confused during
reassembly. (Tip: Use one plastic beaker per pipette).
After autoclaving
Cool the pipette down to room temperature and leave to dry.
For 5 mL / 10 mL pipettes: the protection filter swells during autoclaving. Slightly compress
the protection filter when inserting it into the tip cone.
The piston does not need to be relubricated after autoclaving. If autoclaving is carried out as
described above, no gravimetric testing or adjustment is necessary.
NOTICE!
NOTICE! Damage to device from incorrect handling.
Do not use any additional disinfectants, decontamination agents or sodium
hypochlorite during autoclaving or UV exposure.
When autoclaving make sure that the temperature does not exceed 121°C.
Before using disinfecting agents or decontaminating agents, test for material
compatibility and check the manufacturer's instructions about chemical resistance.
Consider also the material of the pipette.
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