Eppendorf Multipette Xstream - Manual (Page 30)

30
Operation
Multipette
® /Repeater ® (X)stream
English (EN)
3. Press the actuate key to aspirate the liquid.
The display will then show:
4. Hold the Multipette stream/Repeater stream over a waste container or the supply
vessel and press the actuate key.
The reverse stroke is performed. This step is not a dispensing step.
5. Hold the Combitip over the first tube into which the liquid is to be dispensed.
6. Press the actuate key to dispense the liquid. Repeat the process for each additional
tube.
The display shows the dispensing volume and the number of the next dispensing step.
After complete dispensing, the display shows:
7. There are two possible variants for continuing execution:
• Renewed aspiration
a Immerse the Combitip into the liquid to be dispensed.
b Press and hold the actuate key for 2 seconds to aspirate the liquid.
• Completely empty the Combitip and remove
a Hold the Multipette stream/Repeater stream over a tube to dispense the residual
liquid.
b Press the actuate key twice to empty the residual liquid.
c Remove the Combitip.
5.7 Aspirating
(only Multipette Xstream/Repeater Xstream)
1. Prepare the Multipette stream/Repeater stream. To prevent the whirling up of sediment
or to observe a phase limit, select a low aspiration speed.
In the Asp mode you aspirate a defined
volume several times consecutively into the
Combitip, for instance, to take a specific
volume from the wells of a plate. The
Asp
mode is performed approximately in
reverse to the
Dis mode. The aspiration
volume, aspiration step and speed can be
changed during aspiration. After aspiration
of the partial volumes you dispense the total
volume into a tube.
next step
Discard
2.Short = empty
1.Short = no step
Long = aspirate
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