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6 sequential dispensing, Sequential dispensing – Eppendorf Multipette Xstream User Manual

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Operation

Multipette

®

/Repeater

®

(X)stream

English (EN)

After complete dispensing, the display shows:

7. There are two possible variants for continuing execution:

Renewed aspiration
a Immerse the Combitip into the liquid to be dispensed.
b Press and hold the actuate key for 2 seconds to aspirate the liquid.

Completely empty the Combitip and remove
a Hold the Multipette stream/Repeater stream over a tube to dispense the residual

liquid.

b Press the actuate key twice to empty the residual liquid.
c Remove the Combitip.

5.6

Sequential dispensing

(only Multipette Xstream/Repeater Xstream)

This mode is particularly suitable for dilution series. Sequential dispensing is carried out
with two different liquids with the same volumes in two directions. Different dilutions can
be prepared for the same total volume. In the

Seq mode, up to 16 different volumes can

be dispensed. You can change the speed during dispensing. The dispensing speed applies
to all dispensing volumes. The number of dispensing steps and the different dispensing
volumes cannot be changed during execution. If the total of all dispensing volumes
exceeds the nominal volume of the Combitip,

"nnnnn" is displayed for the subsequent

dispensing steps. We recommend to delete these dispensing steps before starting
dispensing to avoid any operating errors. To do this, reduce the number of dispensing
steps in the

Set steps parameter.

1. Prepare the Multipette stream/Repeater stream.

2. Immerse the Combitip into the liquid to be aspirated.

2.Short = empty

1.Short = no step

Long = aspirate

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