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5 automatic dispensing, Automatic dispensing – Eppendorf Multipette Xstream User Manual

Page 28

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28

Operation
Multipette

®

/Repeater

®

(X)stream

English (EN)

7. There are two possible variants for continuing execution:

Renewed aspiration
a Immerse the Combitip into the liquid to be dispensed.
b Press and hold the actuate key for 2 seconds to aspirate the liquid.

Empty the Combitip fully and remove
a Hold the Multipette stream/Repeater stream over a tube to dispense the residual

liquid.

b Press the actuate key twice to empty the residual liquid.
c Remove the Combitip.

5.5

Automatic dispensing

1. Prepare the Multipette stream/Repeater stream (see p. 25). When filling plates with

96 wells we recommend you set the interval time between 0.6 s and 2 s, depending on
the liquid.

2. Immerse the Combitip into the liquid to be aspirated.

3. Press the actuate key.

Liquid is aspirated.
The display will then show:

4. Hold the Multipette stream/Repeater stream over a waste container or the supply

vessel and press the actuate key. The reverse stroke is performed. This step is not a
dispensing step.

5. Hold the Combitip over the first tube into which the liquid is to be dispensed.

6. Press and hold the actuate key to aspirate the liquid automatically. Move the Multipette

stream/Repeater stream to the next tube within the interval time set previously. If the
actuate key is released, the next dispensing steps will not be carried out. You can
continue dispensing afterwards by pressing and holding the actuate key.

The

Ads mode is carried out automatically if

you press and hold the actuate key. For
longer dispensing series (filling plates) in
particular, this mode is more convenient
and quicker than the

Dis mode. The

dispensing volume, the dispensing speed
and the interval time can be changed during
dispensing.

2.Short = empty

1.Short = no step

Long = aspirate

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