Techne 3PrimeG - Manual (Page 36)

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• Touch the A C G T buttons to enter the primer
sequence.
• Touch the arrow to delete the last character.
• Use the < and > keys to scroll through the
sequence.
• Touch Back to return to the previous screen
followed by OK.
• Enter the final primer concentration in the PCR mix.
• Enter the final monovalent salt concentration of the
PCR mix.
• Touch Calculate to calculate the T m
of the primer.
• To check the T m
of another primer with the same
primer and salt concentrations, touch Back and
enter the new primer sequence.
• Touch Reset to clear the entries.
• Touch Back to return to the Settings menu.
• Once all the settings and defaults have been set as required, touch Save to save the changes and
return to the Home Screen.
• If you do not wish to change any settings, touch Cancel to return to the Home Screen.