Techne 3PrimeG - Manual (Page 20)

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GENERAL SOFTWARE FEATURES
INTRODUCTION
The 3 Prime software allows the user to quickly and easily create thermal cycling programs using a
simple and clear touch screen format. The software is structured into three modules which are
accessed from the Home Screen. These allow the user to:
• Run an existing program, selecting from templates or
programs saved on the unit.
• Create a new program.
• Set the instrument and program defaults and
preferences. Includes calculator tools.
TEMPLATE PROGRAMS
To help with programming, a number of program templates are installed on the unit which are
available to copy and edit or can be run directly without changes. Details of the installed program
templates are given below.
Instrument and program defaults
Parameter 2 Step Template 3 Step Template RT-PCR Template
Heated Lid 105ºC 105ºC 105ºC
Heated lid before program On On On
Sample cooling On On On
Pause before program Off Off Off
Initial denaturation 94ºC, 05m00s 94ºC, 05m00s 94ºC, 05m00s
Hot start Off Off Off
Final extension 72ºC, 05m00s 72ºC, 05m00s 72ºC, 07m00s
Final hold 10ºC 10ºC 10ºC
Thermal cycling conditions
2 Step Template 3 Step Template RT-PCR Template
Stage 1
Number of cycles
30 30 1
Step 1 94.0ºC, 00m30s 94.0ºC, 00m30s 45.0ºC, 40m00s
Step 2 60.0ºC, 01m00s 55.0ºC, 00m30s 94.0ºC, 05m00s
Step 3 72.0ºC, 00m30s
Stage 2
Number of cycles
40
Step 1 94.0ºC, 01m00s
Step 2 55.0ºC, 00m50s
Step 3 72.0ºC, 01m00s
The Ice Bucket and Ligation programs are simple temperature holds, with the former having an infinite
hold at 10ºC and the latter at 15ºC. In both of these programs the heated lid is switched off and the
initial denaturation, hot start and final extension functions disabled.