Techne 3PrimeG - Manual (Page 35)

34
SERVICE SETTINGS
The service settings are for the use of authorised
service engineers only. A service engineer’s password
is required to access these settings.
GRADIENT CALCULATOR ( 3 P RIME G ONLY)
The Gradient Calculator can be used to calculate the temperature for each column of the block for a
user-specified temperature and gradient range.
• Touch the Target Temperature button and enter
the required temperature.
• Touch OK.
• Touch the Temperature Gradient button and enter
the required gradient range followed by OK.
• Touch OK.
The calculated temperatures for each of the columns of
the block will now be displayed.
• Touch Back to return to the main menu
OLIGO T
M
CALCULATOR
The oligo T
m
calculator can be used to estimate the T
m
of the PCR primers and help in selecting an
appropriate annealing temperature for the thermal cycling program.
• Touch Enter The Oligo Sequence in order to input
the sequence of the primer.
• Touch the white DNA Base Entry button.