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5 collecting spectra and manipulating data, 1 overview, 2 collecting spectra – Ocean Optics DTS User Manual

Page 37

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Polychromix DTS

TM

Near-Infrared Spectrometer

5-1

Operator’s Manual

Document Number 04-91-0201-00 Rev A

5 Collecting Spectra

and Manipulating
Data

5.1

Overview

This chapter demonstrates how the operator uses the Polychromix
SpectralCode application software program to collect spectra, manipulate
data, and store and retrieve data. The material in this chapter should be used
in conjunction with Chapter 6, which describes sample handling and related
issues.

NOTE: The general approach for collecting spectra and manipulating
data using SpectralCode is identical for all types of data (absorbance,
transmittance and reflectance). For the sake of brevity, only the
collection and processing of transmittance spectra are presented.

5.2

Collecting Spectra

5.2.1

Collecting the Background Spectrum

To Collect the Background Spectrum:

a) Place the blank in the sample compartment (e.g. an empty cuvette)

b) Press the

button

c) Press the

button to collect the spectrum (Figure 5-1).