Olympus IX81 - Manual (Page 45)

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IX81
6 Adjusting the Illumination
Correct illumination is more crucial for photomicrography than for observation because flawless pictures cannot be
obtained unless the illuminating light is properly adjusted. To avoid uneven illumination, especially with high contrast
films, adjust the illuminating light by carefully following the observation procedure.
7 Influence of Radial Heat of Illumination
Even at optimum illumination setting for observation and photomicrography, the illuminating light will generate considerable
radiant heat, which may damage living specimens.
In case of DIC observation, special caution should be exercised in the following points.
· Reduce light intensity for observation as low as possible.
· Use additional heat barrier filters for observation.
· For time-lapse photography, synchronize the on/off of the light bulb with the exposure.
8 Setting the Filter and Lamp Intensity
Set the lamp intensity and filters in accordance with the film to be used.
Film Filter Light Intensity Control Button
Daylight color film 45LBD-IF Camera symbol position
Tungsten type color film 45LBT Camera symbol position
B&W film 43IF550-W45 Other position than camera symbol position
# To match your preference in color rendition, make test exposures for determining the lamp intensity.
}When the IF550 filter is not used for color photography of phase contrast images, a neutral color is obtained by combining
LBD filters and setting the lamp light intensity at around the lower limit of the camera symbol.