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2 differential interference contrast observation, Ix81 – Olympus IX81 User Manual

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IX81

5-2 Differential Interference Contrast Observation

}If a plastic petri dish is used, the normal optical performance of DIC observation cannot be manifested due to the polarization

characteristic of the petri dish. So use a glass petri dish.

}For simultaneous observation with reflected fluorescence observation, refer to the separate instruction manual.
}DIC optical elements, a DIC slider, analyzer and polarizer are required for DIC observation.

1

DIC Optical Elements, Applicable Objectives and DIC Sliders

With the IX2-LWUCD or IX2-LWUCDA2 Condenser

(Note) The IX-ULWCD condenser cannot be used for DIC observation.
}Insert a small optical element (one of the optical elements

inside ( ) in the following table) in the 30 mm position and
other optical element (large) in the 38 mm position.

DIC Slider

Applicable Objective

U-DICT

U-DICTS

Shift Type

U-DICTHC

High Contrast Type

U-DICTHR

High Resolution Type

: To be used in the BFP1 position of the DIC slider.

UIS2 Series

UPlanSApo

10X2

( IX2-DIC10)

( IX2-DIC10)

–––

–––

20X

( IX2-DIC20)

( IX2-DIC20)

( IX2-DIC20HC)

( IX2-DIC20HR)

20XO
40X2

IX2-DIC40

IX2-DIC40

IX2-DIC40HC

IX2-DIC40HR

60XO

–––

IX2-DIC60

–––

–––

60XW

IX2-DIC60

IX2-DIC60

–––

–––

100XO

IX2-DIC100

IX2-DIC100

–––

–––

PlanApoN

60XO

–––

IX2-DIC60

–––

–––

UPlanFLN

10X2

( IX2-DIC10)

( IX2-DIC10)

–––

–––

20X

( IX2-DIC20)

( IX2-DIC20)

( IX2-DIC20HC)

( IX2-DIC20HR)

40X

IX2-DIC40

IX2-DIC40

IX2-DIC40HC

IX2-DIC40HR

40XO

–––

IX2-DIC40

IX2-DIC40HC

IX2-DIC40HR

60X

IX2-DIC60

IX2-DIC60

–––

–––

60XOI

IX2-DIC60

IX2-DIC60

–––

–––

100XO2

IX2-DIC100

IX2-DIC100

–––

–––

100XOI2

LUCPlanFLN

20X

( IX2-DIC20)

( IX2-DIC20)

( IX2-DIC20HC)

( IX2-DIC20HR)

40X

IX2-DIC40

IX2-DIC40

IX2-DIC40HC

IX2-DIC40HR

60X

IX2-DIC60

IX2-DIC60

–––

–––