2 differential interference contrast observation, Ix81 – Olympus IX81 User Manual
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IX81
5-2 Differential Interference Contrast Observation
}If a plastic petri dish is used, the normal optical performance of DIC observation cannot be manifested due to the polarization
characteristic of the petri dish. So use a glass petri dish.
}For simultaneous observation with reflected fluorescence observation, refer to the separate instruction manual.
}DIC optical elements, a DIC slider, analyzer and polarizer are required for DIC observation.
1
DIC Optical Elements, Applicable Objectives and DIC Sliders
With the IX2-LWUCD or IX2-LWUCDA2 Condenser
(Note) The IX-ULWCD condenser cannot be used for DIC observation.
}Insert a small optical element (one of the optical elements
inside ( ) in the following table) in the 30 mm position and
other optical element (large) in the 38 mm position.
DIC Slider
Applicable Objective
U-DICT
U-DICTS
Shift Type
U-DICTHC
High Contrast Type
U-DICTHR
High Resolution Type
: To be used in the BFP1 position of the DIC slider.
UIS2 Series
UPlanSApo
10X2
( IX2-DIC10)
( IX2-DIC10)
–––
–––
20X
( IX2-DIC20)
( IX2-DIC20)
( IX2-DIC20HC)
( IX2-DIC20HR)
20XO
40X2
IX2-DIC40
IX2-DIC40
IX2-DIC40HC
IX2-DIC40HR
60XO
–––
IX2-DIC60
–––
–––
60XW
IX2-DIC60
IX2-DIC60
–––
–––
100XO
IX2-DIC100
IX2-DIC100
–––
–––
PlanApoN
60XO
–––
IX2-DIC60
–––
–––
UPlanFLN
10X2
( IX2-DIC10)
( IX2-DIC10)
–––
–––
20X
( IX2-DIC20)
( IX2-DIC20)
( IX2-DIC20HC)
( IX2-DIC20HR)
40X
IX2-DIC40
IX2-DIC40
IX2-DIC40HC
IX2-DIC40HR
40XO
–––
IX2-DIC40
IX2-DIC40HC
IX2-DIC40HR
60X
IX2-DIC60
IX2-DIC60
–––
–––
60XOI
IX2-DIC60
IX2-DIC60
–––
–––
100XO2
IX2-DIC100
IX2-DIC100
–––
–––
100XOI2
LUCPlanFLN
20X
( IX2-DIC20)
( IX2-DIC20)
( IX2-DIC20HC)
( IX2-DIC20HR)
40X
IX2-DIC40
IX2-DIC40
IX2-DIC40HC
IX2-DIC40HR
60X
IX2-DIC60
IX2-DIC60
–––
–––