Bio-Rad SmartSpec Plus Spectrophotometer - Manual (Page 39)

• If you have varying numbers of replicates for the different standards, press
Select to toggle from Yes to No and press Enter. Skip ahead to b. If you have
the same number of replicates for each standard, press Enter.
a. If there are the same number of replicates for each standard. You
can have as many a nine replicates for each standard
• Use the number keys to input the number of standard replicates and
press Enter.
Now SmartSpec Plus will prompt for the concentration of the first standard and then for
units of concentration.
• Use the number keys to input the concentration of standard #1 and press Enter.
You may enter concentrations with as many as eight significant digits, but all results are
reported with four significant digits because SmartSpec Plus absorbance data are mea-
sured to four significant digits.
Once you have selected the units of concentration, SmartSpec Plus will begin to collect
absorbance measurements on the replicates of standard #1. After all replicates of standard #1
are read, then you will be asked for the concentration of standard #2, and then directed to
input the replicates of standard #2. SmartSpec Plus will continue in this manner until all
replicates of all standards are measured.
• Insert the cuvette with the first replicate of standard #1 into SmartSpec Plus and press
Read Sample.
SmartSpec Plus will continue to prompt for each additional replicate of standard #1 until
all are read.
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•
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Enter the number of
standard replicates: ___
Enter concentration of
standard #1: _________
Insert Rep #2 of Std #1
and PRESS Read Sample
Insert Rep #9 of Std #2
and PRESS Read Sample
Insert Rep 1 of Std #1
and PRESS Read Sample